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### General settings
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In the [file properties dialog](https://bio3d.colorado.edu/SerialEM/hlp/html/hidd_fileproperties.htm), make sure that the single images (not frames) will be saved as MRC and that the "Save extra information in a '.mdoc' metadata file" is checked. This is required for the BISECT python program to work.
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### Setting up the load dose mode presets
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#### Search:
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Search is used to acquire the square maps.
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* The Search magnification depends on what kind of sample is being acquired.
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* If the goal is to acquire tilt series on an evenly distributed sample, a Search magnification that covers the whole square is enough.
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* If the goal is to target precise parts of holes or cells, a search magnification in SA mode will maximize precision.
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#### View:
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View is used for the eucentric height and low mag tracking.
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* Make sure that View is at a magnification that is high enough to see only a few holes (~3-4 um field of view)
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* To speed up tracking calculations, use a binning of 4 for saving the view images.
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#### Record:
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Record is used for the acquisition.
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* Set up Record to the magnification and desired dose conditions.
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* To speed up calculations, use a binning of 2 for saving the record images.
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* This will not affect the resolution at which the frames are saved.
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#### Trial and Focus:
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Trial is used for high-mag tracking.
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* Set up Trial as a copy of Record
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* Make sure Trial and Autofocus areas are on the tilt axis click off inter-axis rotation
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* If working on UltrAUfoil or other gold mesh grid, set trial area to clip the edge of a hole.
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* This is important for tracking.
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* To speed up calculations, use binning 2 for Trial.
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* Trial should have a dose that is high enough for precise alignment. Usually higher than the record dose.
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* As per SerialEM documentation, a binning of 4 should be used for autofocus.
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